What is the catalog number for GFAP ELISA Kit (Rat)?

The catalog number for GFAP ELISA Kit (Rat) is OKEH00112-96W.

What sizes are available for GFAP ELISA Kit (Rat)?

GFAP ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is GFAP ELISA Kit (Rat) in stock?

GFAP ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.

GFAP ELISA Kit (Rat)

Selected Variant

OKEH00112-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Glial fibrillary acidic protein

Gene Aliases

Glial fibrillary acidic protein; glial fibrillary acidic protein alpha; glial fibrillary acidic protein delta; intermediate filament.

Gene ID

24387

Accession Number

NP_058705

Reactivity

Rat, Rattus norvegicus

Target

Glial fibrillary acidic protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GFAP ELISA Kit (Rat) (OKEH00112) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GFAP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GFAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GFAP captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

31.2-2000pg/mL

Sensitivity

7.8 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

50 kDa

Specificity

Natural and recombinant Rat Glial fibrillary acidic protein

Protein Length

430

NCBI Gene Symbol

Gfap

Protein Name

P47819

Gene Name URL

Gfap

Nucleotide Accession Number

NM_017009

Price

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