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IGFBP1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Insulin like growth factor binding protein 1

Gene Aliases

AFBP; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; IGF-BP25; insulin-like growth factor-binding protein 1; placental protein 12; PP12.

Gene ID

3484

Accession Number

NP_000587

Reactivity

Homo sapiens, Human

Target

Insulin-like growth factor-binding protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IGFBP1 ELISA Kit (Human) (OKEH00083) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

10.2 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

28 kDa

Specificity

Natural and recombinant Human Insulin-like growth factor-binding protein 1

Protein Length

259

NCBI Gene Symbol

IGFBP1

Protein Name

P08833

Gene Name URL

IGFBP1

Nucleotide Accession Number

NM_000596

Frequently Asked Questions

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