IFNG ELISA Kit (Pig)
Product Specifications
CAS Number
7732-18-5
Gene Name
Interferon gamma
Gene Aliases
IFN-gamma; interferon gamma.
Gene ID
396991
Accession Number
NP_999113
Reactivity
Porcine, Sus scrofa
Target
Interferon gamma
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IFNG ELISA Kit (Pig) (OKEH00079) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFNG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IFNG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
9.3 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Specificity
Natural and recombinant Pig Interferon gamma
Protein Length
166
NCBI Gene Symbol
IFNG
Protein Name
P17803
Gene Name URL
IFNG
Nucleotide Accession Number
NM_213948
Frequently Asked Questions
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