HGF ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Hepatocyte growth factor
Gene Aliases
DFNB39; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor; hepatocyte growth factor (hepapoietin A; scatter factor) ; hepatopoietin-A; HGFB; HPTA; lung fibroblast-derived mitogen; Scatter factor; SF.
Gene ID
3082
Accession Number
NP_000592
Reactivity
Homo sapiens, Human
Target
Hepatocyte growth factor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HGF ELISA Kit (Human) (OKEH00071) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HGF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HGF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HGF captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
Sensitivity
19.5 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
83 kDa
Specificity
Natural and recombinant Human Hepatocyte growth factor
Protein Length
728
NCBI Gene Symbol
HGF
Protein Name
P14210
Gene Name URL
HGF
Nucleotide Accession Number
NM_000601
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
