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PPBP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Pro-platelet basic protein

Gene Aliases

Beta-TG; beta-thromboglobulin; B-TG1; chemokine (C-X-C motif) ligand 7; connective tissue-activating peptide III; CTAP3; CTAPIII; CTAP-III; CXC chemokine ligand 7; C-X-C motif chemokine 7; CXCL7; LA-PF4; LDGF; leukocyte-derived growth factor; low-affinity platelet factor IV; macrophage-derived growth factor; MDGF; NAP-2; neutrophil-activating peptide 2; PBP; platelet basic protein; SCYB7; small inducible cytokine B7; small inducible cytokine subfamily B, member 7; Small-inducible cytokine B7; TC1; TC2; TGB; TGB1; THBGB; THBGB1; thrombocidin 1; thrombocidin 2; thromboglobulin, beta-1.

Gene ID

5473

Accession Number

NP_002695

Reactivity

Homo sapiens, Human

Target

Platelet basic protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PPBP ELISA Kit (Human) (OKEH00061) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPBP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPBP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PPBP captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

3.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

14 kDa

Specificity

Natural and recombinant Human Platelet basic protein

Protein Length

128

NCBI Gene Symbol

PPBP

Protein Name

P02775

Gene Name URL

PPBP

Nucleotide Accession Number

NM_002704

Frequently Asked Questions

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