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FLT3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Fms related receptor tyrosine kinase 3

Gene Aliases

CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK2; FLK-2; fms related tyrosine kinase 3; fms-like tyrosine kinase 3; growth factor receptor tyrosine kinase type III; receptor-type tyrosine-protein kinase FLT3; stem cell tyrosine kinase 1; STK1.

Gene ID

2322

Accession Number

NP_004110

Reactivity

Homo sapiens, Human

Target

Receptor-type tyrosine-protein kinase FLT3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FLT3 ELISA Kit (Human) (OKEH00059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FLT3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FLT3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FLT3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into Serum and Plasma = 93%

Sensitivity

21 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

113 kDa

Specificity

Natural and recombinant Human Receptor-type tyrosine-protein kinase FLT3

Protein Length

993

NCBI Gene Symbol

FLT3

Protein Name

P36888

Gene Name URL

FLT3

Nucleotide Accession Number

NM_004119

Frequently Asked Questions

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