FLT3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fms related receptor tyrosine kinase 3
Gene Aliases
CD135; CD135 antigen; fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK2; FLK-2; fms related tyrosine kinase 3; fms-like tyrosine kinase 3; growth factor receptor tyrosine kinase type III; receptor-type tyrosine-protein kinase FLT3; stem cell tyrosine kinase 1; STK1.
Gene ID
2322
Accession Number
NP_004110
Reactivity
Homo sapiens, Human
Target
Receptor-type tyrosine-protein kinase FLT3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FLT3 ELISA Kit (Human) (OKEH00059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FLT3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FLT3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FLT3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 93%
Sensitivity
21 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
113 kDa
Specificity
Natural and recombinant Human Receptor-type tyrosine-protein kinase FLT3
Protein Length
993
NCBI Gene Symbol
FLT3
Protein Name
P36888
Gene Name URL
FLT3
Nucleotide Accession Number
NM_004119
Frequently Asked Questions
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