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FGF1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Fibroblast growth factor 1

Gene Aliases

Acidic fibroblast growth factor; AFGF; beta-endothelial cell growth factor; ECGF; ECGFA; ECGFB; ECGF-beta; Endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; FGF-1; FGFA; FGF-alpha; fibroblast growth factor 1; fibroblast growth factor 1 (acidic) ; GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1.

Gene ID

2246

Accession Number

NP_000791

Reactivity

Homo sapiens, Human

Target

Fibroblast growth factor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FGF1 ELISA Kit (Human) (OKEH00051) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGF1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGF1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGF1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

21 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

17 kDa

Specificity

Natural and recombinant Human Fibroblast growth factor 1

Protein Length

155

NCBI Gene Symbol

FGF1

Protein Name

P05230

Gene Name URL

FGF1

Nucleotide Accession Number

NM_000800

Frequently Asked Questions

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