FASLG ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fas ligand
Gene Aliases
Apoptosis (APO-1) antigen ligand 1 (Fas antigen ligand) ; Apt1Lg1; CD95 ligand; CD95-L; CD95L protein; Fas antigen ligand; Fas ligand (TNF superfamily, member 6) ; Fasl; Tnfsf6; Tnlg1a; Tumor necrosis factor (ligand) superfamily, member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand) ; tumor necrosis factor ligand 1a; tumor necrosis factor ligand superfamily member 6.
Gene ID
25385
Accession Number
NP_037040
Reactivity
Rat, Rattus norvegicus
Target
Tumor necrosis factor ligand superfamily member 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FASLG ELISA Kit (Rat) (OKEH00050) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FASLG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FASLG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FASLG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Rat Tumor necrosis factor ligand superfamily member 6
Protein Length
278
NCBI Gene Symbol
Faslg
Protein Name
P36940
Gene Name URL
Faslg
Nucleotide Accession Number
NM_012908
Frequently Asked Questions
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