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FASLG ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Fas ligand

Gene Aliases

Apoptosis (APO-1) antigen ligand 1 (Fas antigen ligand) ; Apt1Lg1; CD95 ligand; CD95-L; CD95L protein; Fas antigen ligand; Fas ligand (TNF superfamily, member 6) ; Fasl; Tnfsf6; Tnlg1a; Tumor necrosis factor (ligand) superfamily, member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand) ; tumor necrosis factor ligand 1a; tumor necrosis factor ligand superfamily member 6.

Gene ID

25385

Accession Number

NP_037040

Reactivity

Rat, Rattus norvegicus

Target

Tumor necrosis factor ligand superfamily member 6

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FASLG ELISA Kit (Rat) (OKEH00050) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FASLG has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FASLG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FASLG captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

31 kDa

Specificity

Natural and recombinant Rat Tumor necrosis factor ligand superfamily member 6

Protein Length

278

NCBI Gene Symbol

Faslg

Protein Name

P36940

Gene Name URL

Faslg

Nucleotide Accession Number

NM_012908

Frequently Asked Questions

More Discoveries

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