FAS ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fas cell surface death receptor
Gene Aliases
ALPS1A; APO-1; Apo-1 antigen; APO-1 cell surface antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; APT1; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6) ; Fas AMA; FAS1; FASLG receptor; FASTM; mutant tumor necrosis receptor superfamily member 6; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6.
Gene ID
355
Accession Number
NP_000034
Reactivity
Homo sapiens, Human
Target
Tumor necrosis factor receptor superfamily member 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FAS ELISA Kit (Human) (OKEH00048) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FAS has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FAS is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FAS captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
24 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
38 kDa
Specificity
Natural and recombinant Human Tumor necrosis factor receptor superfamily member 6
Protein Length
335
NCBI Gene Symbol
FAS
Protein Name
P25445
Gene Name URL
FAS
Nucleotide Accession Number
NM_000043
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
GTR15110840 1 Vial
Lentiviral human ORM1 shRNA (UAS) - Lentiviral human ORM1 shRNA (UAS, GFP) (25)
Ask
View Details 
MBS6265906-01 0.1 mL
Matrix Metalloproteinase 3 (MMP-3, Stromelysin-1, SL-1, Transin) (BSA & Azide Free) (AP)
Ask
View Details MBS6265906-02 5x 0.1 mL
Matrix Metalloproteinase 3 (MMP-3, Stromelysin-1, SL-1, Transin) (BSA & Azide Free) (AP)
Ask
View Details