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SELE ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Selectin E

Gene Aliases

CD62 antigen-like family member E; CD62E; ELAM; ELAM1; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule 1; ESEL; E-selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2.

Gene ID

6401

Accession Number

NP_000441

Reactivity

Homo sapiens, Human

Target

E-selectin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SELE ELISA Kit (Human) (OKEH00046) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SELE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SELE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SELE captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

67 kDa

Specificity

Natural and recombinant Human E-selectin

Protein Length

610

NCBI Gene Symbol

SELE

Protein Name

P16581

Gene Name URL

SELE

Nucleotide Accession Number

NM_000450

Frequently Asked Questions

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