CCL11 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
C-C motif chemokine ligand 11
Gene Aliases
C-C motif chemokine 11; chemokine (C-C motif) ligand 11; eosinophil chemotactic protein; eotaxin; Scya11; small inducible cytokine subfamily A11; small-inducible cytokine A11.
Gene ID
29397
Accession Number
NP_062078
Reactivity
Rat, Rattus norvegicus
Target
Eotaxin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CCL11 ELISA Kit (Rat) (OKEH00040) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL11 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
7.92 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Specificity
Natural and recombinant Rat Eotaxin
Protein Length
97
NCBI Gene Symbol
Ccl11
Protein Name
P97545
Gene Name URL
Ccl11
Nucleotide Accession Number
NM_019205
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC741577-500 1x 500 µL
Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker) (KRTL/1577R), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
