BMPR1A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Bone morphogenetic protein receptor type 1A
Gene Aliases
10q23del; activin A receptor, type II-like kinase 3; activin receptor-like kinase 3; ACVRLK3; ALK3; ALK-3; BMP type-1A receptor; BMPR-1A; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292; serine/threonine-protein kinase receptor R5; SKR5.
Gene ID
657
Accession Number
NP_004320
Reactivity
Homo sapiens, Human
Target
Bone morphogenetic protein receptor type-1A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology BMPR1A ELISA Kit (Human) (OKEH00029) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMPR1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMPR1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMPR1A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
Sensitivity
0.11 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Specificity
Natural and recombinant Human Bone morphogenetic protein receptor type-1A
Protein Length
532
NCBI Gene Symbol
BMPR1A
Protein Name
P36894
Gene Name URL
BMPR1A
Nucleotide Accession Number
NM_004329
Frequently Asked Questions
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