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BMP2 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Bone morphogenetic protein 2

Gene Aliases

AI467020; Bmp; Bmp2a; bone morphogenetic protein 2; bone morphogenetic protein 2A.

Gene ID

12156

Accession Number

NP_031579

Reactivity

Mouse, Mus musculus

Target

Bone morphogenetic protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology BMP2 ELISA Kit (Mouse) (OKEH00026) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMP2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%

Sensitivity

31.25 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

45 kDa

Specificity

Natural and recombinant Mouse Bone morphogenetic protein 2

Protein Length

394

NCBI Gene Symbol

Bmp2

Protein Name

P21274

Gene Name URL

Bmp2

Nucleotide Accession Number

NM_007553

Frequently Asked Questions

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