PLIN4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Perilipin 4

Gene Aliases

Adipocyte protein S3-12; mKIAA1881; perilipin-4; plasma membrane associated protein, S3-12; S3-1; S3-12.

Gene ID

57435

Accession Number

NP_065593.2

Reactivity

Mouse|Mus musculus

Target

May play a role in triacylglycerol packaging into adipocytes. May function as a coat protein involved in the biogenesis of lipid droplets.

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology PLIN4 ELISA Kit (Mouse) (OKDD00698) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for PLIN4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PLIN4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PLIN4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156-10ng/mL

Sensitivity

<0.055 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

139 kDa

Notes

Formerly GWB-KBBWR7

Specificity

This assay has high sensitivity and excellent specificity for detection of PLIN4. No significant cross-reactivity or interference between PLIN4 and analogues was observed.