PF4 ELISA Kit (Rat)
Product Specifications
Reproducibility
Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV_x005F Mean Inter-Assay: CV _x005F Mean Inter-Assay: CV Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Name
Platelet factor 4
Gene Aliases
Chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; PF-4; Pf4a; platelet factor 4; RATPF4A.
Gene ID
360918
Accession Number
NP_001007730.1
Reactivity
Rat|Rattus norvegicus
Target
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
Type
ELISA Kit
Applications
Enzyme-linked immunosorbent assay
Assay Principle
Aviva Systems Biology PF4 ELISA Kit (Rat) (OKDD00622) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for PF4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PF4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PF4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
< 0.128 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Notes
Formerly GWB-KBBFQ3
Specificity
This assay has high sensitivity and excellent specificity for detection of PF4._x005F No significant cross-reactivity or interference between PF4 and analogues was observed._x005F
Protein Length
105
NCBI Gene Symbol
Pf4
Protein Name
Platelet factor 4
Gene Name URL
Pf4
Nucleotide Accession Number
NM_001007729.1
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