What is the catalog number for PF4 ELISA Kit (Rat)?

The catalog number for PF4 ELISA Kit (Rat) is OKDD00622-96W.

What sizes are available for PF4 ELISA Kit (Rat)?

PF4 ELISA Kit (Rat) is available in the following size(s): 96 Tests.

Is PF4 ELISA Kit (Rat) in stock?

PF4 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

PF4 ELISA Kit (Rat)

Product Specifications

Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV_x005F Mean Inter-Assay: CV _x005F Mean Inter-Assay: CV Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Platelet factor 4

Gene Aliases

Chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; PF-4; Pf4a; platelet factor 4; RATPF4A.

Gene ID

360918

Accession Number

NP_001007730.1

Reactivity

Rat|Rattus norvegicus

Target

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology PF4 ELISA Kit (Rat) (OKDD00622) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for PF4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PF4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PF4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids.

Detection Range

0.312-20ng/mL

Sensitivity

< 0.128 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

11 kDa

Notes

Formerly GWB-KBBFQ3

Specificity

This assay has high sensitivity and excellent specificity for detection of PF4._x005F No significant cross-reactivity or interference between PF4 and analogues was observed._x005F