MCP1 ELISA Kit (Monkey)

Product Specifications

Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively. Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate. Mean Intra-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

C-C motif chemokine ligand 2

Gene Aliases

C-C motif chemokine 2; MCP1; MCP-1; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; SCYA2; Small-inducible cytokine A2.

Gene ID

102135739

Accession Number

NP_001271801.1

Reactivity

Macaca fascicularis|Rhesus Monkey

Target

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Binds to CCR2 and CCR4 (By similarity) .

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology MCP1 ELISA Kit (Monkey) (OKDD00615) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for MCP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MCP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MCP1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

Detection Range

0.156-10ng/mL

Sensitivity

<0.054 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

11 kDa

Notes

Formerly GWB-KBBUL9

Specificity

This assay has high sensitivity and excellent specificity for detection of MCP1. No significant cross-reactivity or interference between MCP1 and analogues was observed.