What is the catalog number for MPI ELISA Kit (Human)?

The catalog number for MPI ELISA Kit (Human) is OKDD00401-96W.

What sizes are available for MPI ELISA Kit (Human)?

MPI ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is MPI ELISA Kit (Human) in stock?

MPI ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

MPI ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level MPI were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level MPI were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV_x005F Inter-Assay: CV _x005F Inter-Assay: CV Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Mannose phosphate isomerase

Gene Aliases

CDG1B; mannose-6-phosphate isomerase; phosphohexomutase; Phosphomannose isomerase; phosphomannose isomerase 1; PMI; PMI1.

Gene ID

4351

Accession Number

NP_001276085.1

Reactivity

Homo sapiens|Human

Target

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology MPI ELISA Kit (Human) (OKDD00401) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MPI has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MPI is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MPI captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates

Detection Range

0.156-10ng/mL

Sensitivity

< 0.061 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

47 kDa

Notes

Formerly GWB-KBBGB8

Specificity

This assay has high sensitivity and excellent specificity for detection of MPI._x005F No significant cross-reactivity or interference between MPI and analogues was observed.