What is the catalog number for MGL ELISA Kit (Human)?

The catalog number for MGL ELISA Kit (Human) is OKDD00391-96W.

What sizes are available for MGL ELISA Kit (Human)?

MGL ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is MGL ELISA Kit (Human) in stock?

MGL ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

MGL ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level MGL were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level MGL were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Monoglyceride lipase

Gene Aliases

HUK5; HU-K5; lysophospholipase homolog; Lysophospholipase-like; MAGL; MGL; monoacylglycerol lipase; monoglyceride lipase.

Gene ID

11343

Accession Number

NP_001003794.1

Reactivity

Homo sapiens|Human

Target

Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity) . Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (PubMed:20079333) .

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology MGL ELISA Kit (Human) (OKDD00391) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MGL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MGL is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MGL captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates

Detection Range

0.156-10ng/mL

Sensitivity

< 0.059 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

33 kDa

Notes

Formerly GWB-KBBCM9

Specificity

This assay has high sensitivity and excellent specificity for detection of MGL. No significant cross-reactivity or interference between MGL and analogues was observed.