What is the catalog number for LAMP1 ELISA Kit (Human)?

The catalog number for LAMP1 ELISA Kit (Human) is OKDD00368-96W.

What sizes are available for LAMP1 ELISA Kit (Human)?

LAMP1 ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is LAMP1 ELISA Kit (Human) in stock?

LAMP1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

LAMP1 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level LAMP1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level LAMP1 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Lysosomal associated membrane protein 1

Gene Aliases

CD107 antigen-like family member A; CD107a; LAMP-1; LAMPA; LGP120; lysosome-associated membrane glycoprotein 1; lysosome-associated membrane protein 1.

Gene ID

3916

Accession Number

NP_005552.3

Reactivity

Homo sapiens|Human

Target

Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology LAMP1 ELISA Kit (Human) (OKDD00368) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LAMP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LAMP1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LAMP1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Tissue homogenates, cell lysates or other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

< 0.059 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

45 kDa

Notes

Formerly GWB-KBBHG8

Specificity

This assay has high sensitivity and excellent specificity for detection of LAMP1. No significant cross-reactivity or interference between LAMP1 and analogues was observed.