What is the catalog number for DLL3 ELISA Kit (Human)?

The catalog number for DLL3 ELISA Kit (Human) is OKDD00231-96W.

What sizes are available for DLL3 ELISA Kit (Human)?

DLL3 ELISA Kit (Human) is available in the following size(s): 96 Tests.

Is DLL3 ELISA Kit (Human) in stock?

DLL3 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

DLL3 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level DLL3 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level DLL3 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Delta like canonical Notch ligand 3

Gene Aliases

Delta3; delta-like 3; delta-like protein 3; drosophila Delta homolog 3; SCDO1.

Gene ID

10683

Accession Number

NP_058637.1

Reactivity

Homo sapiens|Human

Target

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity) .

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology dLL3 ELISA Kit (Human) (OKDD00231) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for dLL3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for dLL3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample dLL3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates

Detection Range

0.156-10ng/mL

Sensitivity

< 0.069 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

65 kDa

Notes

Formerly GWB-KBBRK5

Specificity

This assay has high sensitivity and excellent specificity for detection of DLL3. No significant cross-reactivity or interference between DLL3 and analogues was observed.