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CAP2 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level CAP2 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level CAP2 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV Inter-Assay: CV

CAS Number

7732-18-5

Gene Name

Cyclase associated actin cytoskeleton regulatory protein 2

Gene Aliases

2810452G09Rik; adenylyl cyclase-associated protein 2; CAP 2; CAP, adenylate cyclase-associated protein, 2.

Gene ID

10486

Accession Number

NP_006357.1

Reactivity

Homo sapiens|Human

Target

May have a regulatory bifunctional role.

Type

ELISA Kit

Applications

Enzyme-linked immunosorbent assay

Assay Principle

Aviva Systems Biology CAP2 ELISA Kit (Human) (OKDD00164) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for CAP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAP2 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CAP2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates

Detection Range

0.156-10ng/mL

Sensitivity

< 0.056 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

53 kDa

Notes

Formerly GWB-KBBLB5

Specificity

This assay has high sensitivity and excellent specificity for detection of CAP2. No significant cross-reactivity or interference between CAP2 and analogues was observed.

Protein Length

477

NCBI Gene Symbol

CAP2

Protein Name

Adenylyl cyclase-associated protein 2

Gene Name URL

CAP2

Nucleotide Accession Number

NM_006366.2

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