RHD ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Rh Blood Group, D Antigen (RHD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Rh Blood Group, D Antigen (RHD) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Rh blood group D antigen

Gene Aliases

Blood group antigen D; blood group protein RHD; blood group Rh (D) polypeptide; CD240D; D antigen (DCS) ; DIIIc; HDFNRH; RH; Rh blood group antigen Evans; Rh blood group C antigen; Rh blood group CcEe antigen; Rh blood group D antigen weak D type 160; Rh blood group, D anitgen; RH polypeptide 2; RH30; Rh4; RHCED; RhD antigen; RhD blood group antigen; RhD polypeptide; RhDCw; RHDel; RHDVA (TT) ; Rhesus blood group antigen D; Rhesus blood group D antigen allele DIII type 7; rhesus D antigen; Rhesus D blood group protein; Rhesus system D polypeptide; RhII; RhK562-II; RhPI; RHPII; RHXIII; truncated Rh blood group D antigen; truncated RhD antigen; truncated rhesus blood group D antigen; truncated rhesus D.

Gene ID

6007

Accession Number

NP_001121163.1

Reactivity

Homo sapiens|Human

Target

May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rh Blood Group, D Antigen (RHD) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rh Blood Group, D Antigen (RHD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rh Blood Group, D Antigen (RHD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rh Blood Group, D Antigen (RHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

1.56-100ng/mL

Sensitivity

< 0.66ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount RHD Microplate 96 Wells (12 x 8 Well strips) RHD Lyophilized Standard 2 100X RHD HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount RHD Microplate 96 Wells (12 x 8 Well strips) RHD Microplate 96 Wells (12 x 8 Well strips) RHD Lyophilized Standard 2 RHD Lyophilized Standard 2 100X RHD HRP-Detector Anitbody 1 x 120 uL 100X RHD HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL