What is the catalog number for LNPEP ELISA Kit (Human)?

The catalog number for LNPEP ELISA Kit (Human) is OKCD09088-96W.

What sizes are available for LNPEP ELISA Kit (Human)?

LNPEP ELISA Kit (Human) is available in the following size(s): 96 Well.

Is LNPEP ELISA Kit (Human) in stock?

LNPEP ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

LNPEP ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Leucyl/Cystinyl Aminopeptidase (LNPEP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Leucyl/Cystinyl Aminopeptidase (LNPEP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Leucyl and cystinyl aminopeptidase

Gene Aliases

Angiotensin IV receptor; AT (4) receptor; CAP; cystinyl aminopeptidase; insulin-regulated aminopeptidase; insulin-regulated membrane aminopeptidase; insulin-responsive aminopeptidase; IRAP; leucyl-cystinyl aminopeptidase; otase; oxytocinase; placental leucine aminopeptidase; PLAP; P-LAP; vasopressinase.

Gene ID

4012

Accession Number

NP_005566.2

Reactivity

Homo sapiens|Human

Target

Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucyl/Cystinyl Aminopeptidase (LNPEP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucyl/Cystinyl Aminopeptidase (LNPEP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucyl/Cystinyl Aminopeptidase (LNPEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Leucyl/Cystinyl Aminopeptidase (LNPEP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

< 0.064ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount LNPEP Microplate 96 Wells (12 x 8 Well strips) LNPEP Lyophilized Standard 2 100X LNPEP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount LNPEP Microplate 96 Wells (12 x 8 Well strips) LNPEP Microplate 96 Wells (12 x 8 Well strips) LNPEP Lyophilized Standard 2 LNPEP Lyophilized Standard 2 100X LNPEP HRP-Detector Anitbody 1 x 120 uL 100X LNPEP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL