What is the catalog number for EPB42 ELISA Kit (Human)?

The catalog number for EPB42 ELISA Kit (Human) is OKCD08750-96W.

What sizes are available for EPB42 ELISA Kit (Human)?

EPB42 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is EPB42 ELISA Kit (Human) in stock?

EPB42 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

EPB42 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Erythrocyte Membrane Protein Band 4.2 (EPB42) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Erythrocyte Membrane Protein Band 4.2 (EPB42) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Erythrocyte membrane protein band 4.2

Gene Aliases

Erythrocyte protein 4.2; erythrocyte surface protein band 4.2; P4.2; PA; protein 4.2; SPH5.

Gene ID

2038

Accession Number

NP_000110.2

Reactivity

Homo sapiens|Human

Target

Probably plays an important role in the regulation of erythrocyte shape and mechanical properties.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythrocyte Membrane Protein Band 4.2 (EPB42) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Erythrocyte Membrane Protein Band 4.2 (EPB42) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythrocyte Membrane Protein Band 4.2 (EPB42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Erythrocyte Membrane Protein Band 4.2 (EPB42) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Tissue homogenates, cell lysates and other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

< 0.053ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount EPB42 Microplate 96 Wells (12 x 8 Well strips) EPB42 Lyophilized Standard 2 100X EPB42 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount EPB42 Microplate 96 Wells (12 x 8 Well strips) EPB42 Microplate 96 Wells (12 x 8 Well strips) EPB42 Lyophilized Standard 2 EPB42 Lyophilized Standard 2 100X EPB42 HRP-Detector Anitbody 1 x 120 uL 100X EPB42 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL