CGB3 MORE ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chorionic Gonadotropin Beta Polypeptide (CGb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chorionic Gonadotropin Beta Polypeptide (CGb) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Chorionic gonadotropin subunit beta 3|chorionic gonadotropin subunit beta 5|chorionic gonadotropin subunit beta 7|chorionic gonadotropin subunit beta 8

CGB; CGB5; CGB6; CGB7; CGB8; CG-beta; CG-beta-a; Choriogonadotropin subunit beta; choriogonadotropin subunit beta 3; choriogonadotropin subunit beta 7; chorionic gonadotrophin chain beta; chorionic gonadotropin beta 3 subunit; chorionic gonadotropin beta 5 subunit; chorionic gonadotropin beta 7 subunit; chorionic gonadotropin beta 8 subunit; chorionic gonadotropin beta chain; chorionic gonadotropin beta subunit 3; chorionic gonadotropin beta subunit 5; chorionic gonadotropin beta subunit 7; chorionic gonadotropin beta subunit 8; chorionic gonadotropin chain beta; chorionic gonadotropin, beta polypeptide; chorionic gonadotropin, beta polypeptide 5; chorionic gonadotropin, beta polypeptide 7; chorionic gonadotropin, beta polypeptide 8; HCG; hCGB; LHB; luteinizing hormone beta subunit.

Beta subunit of the human chorionic gonadotropin (hCG) . hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH) . The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role for pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.|Beta subunit of the human chorionic gonadotropin (hCG) . hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH) . The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chorionic Gonadotropin Beta Polypeptide (CGb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Amount CGB3 Microplate 96 Wells (12 x 8 Well strips) CGB3 Lyophilized Standard 2 100X CGB3 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount CGB3 Microplate 96 Wells (12 x 8 Well strips) CGB3 Microplate 96 Wells (12 x 8 Well strips) CGB3 Lyophilized Standard 2 CGB3 Lyophilized Standard 2 100X CGB3 HRP-Detector Anitbody 1 x 120 uL 100X CGB3 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL