What is the catalog number for FBLN5 ELISA Kit (Human)?

The catalog number for FBLN5 ELISA Kit (Human) is OKCD08402-96W.

What sizes are available for FBLN5 ELISA Kit (Human)?

FBLN5 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is FBLN5 ELISA Kit (Human) in stock?

FBLN5 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

FBLN5 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Fibulin 5 (FBLN5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Fibulin 5 (FBLN5) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Fibulin 5

Gene Aliases

ADCL2; ARCL1A; ARMD3; DANCE; developmental arteries and neural crest EGF-like protein; embryonic vascular EGF-like repeat-containing protein; EVEC; FIBL-5; fibulin-5; HNARMD; testis tissue sperm-binding protein Li 75n; UP50; urine p50 protein.

Gene ID

10516

Accession Number

NP_006320.2

Reactivity

Homo sapiens|Human

Target

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (PubMed:18185537) . Stabilizes and organizes elastic fibers in the skin, lung and vasculature (By similarity) . Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling (PubMed:10428823) . May act as an adapter that mediates the interaction between FBN1 and ELN (PubMed:17255108) .

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 5 (FBLN5) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 5 (FBLN5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 5 (FBLN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibulin 5 (FBLN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, urine and other biological fluids

Detection Range

15.6-1,000pg/mL

Sensitivity

< 6.2pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount FBLN5 Microplate 96 Wells (12 x 8 Well strips) FBLN5 Lyophilized Standard 2 100X FBLN5 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount FBLN5 Microplate 96 Wells (12 x 8 Well strips) FBLN5 Microplate 96 Wells (12 x 8 Well strips) FBLN5 Lyophilized Standard 2 FBLN5 Lyophilized Standard 2 100X FBLN5 HRP-Detector Anitbody 1 x 120 uL 100X FBLN5 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL