What is the catalog number for JUP ELISA Kit (Human)?

The catalog number for JUP ELISA Kit (Human) is OKCD08040-96W.

What sizes are available for JUP ELISA Kit (Human)?

JUP ELISA Kit (Human) is available in the following size(s): 96 Well.

Is JUP ELISA Kit (Human) in stock?

JUP ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

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Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

JUP ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Junction Plakoglobin (JUP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Junction Plakoglobin (JUP) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Junction plakoglobin

Gene Aliases

Catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; CTNNG; desmoplakin III; desmoplakin-3; desmosomal protein 3; DP3; DPIII; junction plakoglobin; PDGB; PG; PKGB.

Gene ID

3728

Accession Number

NP_002221.1

Reactivity

Homo sapiens|Human

Target

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity) .

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Detection Method

Colorimetric

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junction Plakoglobin (JUP) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junction Plakoglobin (JUP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junction Plakoglobin (JUP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Junction Plakoglobin (JUP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

< 0.056ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount JUP Microplate 96 Wells (12 x 8 Well strips) JUP Lyophilized Standard 2 100X JUP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Component Amount Component Amount JUP Microplate 96 Wells (12 x 8 Well strips) JUP Microplate 96 Wells (12 x 8 Well strips) JUP Lyophilized Standard 2 JUP Lyophilized Standard 2 100X JUP HRP-Detector Anitbody 1 x 120 uL 100X JUP HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 2 mL TMB Substrate 1 x 2 mL Stop Solution 1 x 20 mL Stop Solution 1 x 20 mL