What is the catalog number for F10 Chemi-Luminescent ELISA Kit (Human)?

The catalog number for F10 Chemi-Luminescent ELISA Kit (Human) is OKCD05555-96W.

What sizes are available for F10 Chemi-Luminescent ELISA Kit (Human)?

F10 Chemi-Luminescent ELISA Kit (Human) is available in the following size(s): 96 Well.

Is F10 Chemi-Luminescent ELISA Kit (Human) in stock?

F10 Chemi-Luminescent ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

F10 Chemi-Luminescent ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Coagulation Factor X (F10) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Coagulation Factor X (F10) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Coagulation factor X

Gene Aliases

Coagulation factor X; factor X; factor Xa; FX; FXA; prothrombinase; Stuart factor; Stuart-Prower factor.

Gene ID

2159

Accession Number

NP_000495.1

Reactivity

Homo sapiens|Human

Target

Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor X (F10) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Coagulation Factor X (F10) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Coagulation Factor X (F10) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Plasma

Detection Range

8.23-6,000pg/mL

Sensitivity

< 3.05pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount F10 Microplate 96 Wells (12 x 8 Well strips) F10 Lyophilized Standard 2 100X F10 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 9 mL Substrate Diluent 1 x 6 mL Component Amount Component Amount F10 Microplate 96 Wells (12 x 8 Well strips) F10 Microplate 96 Wells (12 x 8 Well strips) F10 Lyophilized Standard 2 F10 Lyophilized Standard 2 100X F10 HRP-Detector Anitbody 1 x 120 uL 100X F10 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 9 mL 100X Luminol Substrate 1 x 9 mL Substrate Diluent 1 x 6 mL Substrate Diluent 1 x 6 mL