What is the catalog number for CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig)?

The catalog number for CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig) is OKCD05507-96W.

What sizes are available for CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig)?

CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig) is available in the following size(s): 96 Well.

Is CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig) in stock?

CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

CCL11 Chemi-Luminescent ELISA Kit (Guinea Pig)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

C-C motif chemokine ligand 11

Gene Aliases

C-C motif chemokine 11; chemokine (C-C motif) ligand 11; eosinophil chemotactic protein; eotaxin; SCYA11; small chemokine (C-C motif) ligand 11; small-inducible cytokine A11.

Gene ID

100135493

Accession Number

NP_001166295.1

Reactivity

Cavia porcellus|Guinea Pig

Target

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Eosinophil Chemotactic Factor (ECF) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Eosinophil Chemotactic Factor (ECF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Eosinophil Chemotactic Factor (ECF) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.37-1,000pg/mL

Sensitivity

< 0.49pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount CCL11 Microplate 96 Wells (12 x 8 Well strips) CCL11 Lyophilized Standard 2 100X CCL11 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 9 mL Substrate Diluent 1 x 6 mL Component Amount Component Amount CCL11 Microplate 96 Wells (12 x 8 Well strips) CCL11 Microplate 96 Wells (12 x 8 Well strips) CCL11 Lyophilized Standard 2 CCL11 Lyophilized Standard 2 100X CCL11 HRP-Detector Anitbody 1 x 120 uL 100X CCL11 HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 9 mL 100X Luminol Substrate 1 x 9 mL Substrate Diluent 1 x 6 mL Substrate Diluent 1 x 6 mL