FGF15 ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 15 (FGF15) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 15 (FGF15) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 15 (FGF15) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Fibroblast Growth Factor 15 (FGF15) and unlabeled High Sensitive Fibroblast Growth Factor 15 (FGF15) (Standards or samples) with the pre-coated antibody specific to High Sensitive Fibroblast Growth Factor 15 (FGF15) . After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 15 (FGF15) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 15 (FGF15) in the sample.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Amount FGF15 Microplate 96 Wells (12 x 8 Well strips) FGF15 Lyophilized Standard 2 100X FGF15 HRP-Detector Anitbody 1 x 120 ÂuL 100X Avidin-HRP Conjugate 1 x 120 ÂuL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount FGF15 Microplate 96 Wells (12 x 8 Well strips) FGF15 Microplate 96 Wells (12 x 8 Well strips) FGF15 Lyophilized Standard 2 FGF15 Lyophilized Standard 2 100X FGF15 HRP-Detector Anitbody 1 x 120 ÂuL 100X FGF15 HRP-Detector Anitbody 1 x 120 ÂuL 100X Avidin-HRP Conjugate 1 x 120 ÂuL 100X Avidin-HRP Conjugate 1 x 120 ÂuL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL