What is the catalog number for RHD ELISA Kit (Human)?

The catalog number for RHD ELISA Kit (Human) is OKCD04533-96W.

What sizes are available for RHD ELISA Kit (Human)?

RHD ELISA Kit (Human) is available in the following size(s): 96 Well.

Is RHD ELISA Kit (Human) in stock?

RHD ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

RHD ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Rh blood group D antigen

Gene Aliases

Blood group antigen D; blood group protein RHD; blood group Rh (D) polypeptide; CD240D; D antigen (DCS) ; DIIIc; HDFNRH; RH; Rh blood group antigen Evans; Rh blood group C antigen; Rh blood group CcEe antigen; Rh blood group D antigen weak D type 160; Rh blood group, D anitgen; RH polypeptide 2; RH30; Rh4; RHCED; RhD antigen; RhD blood group antigen; RhD polypeptide; RhDCw; RHDel; RHDVA (TT) ; Rhesus blood group antigen D; Rhesus blood group D antigen allele DIII type 7; rhesus D antigen; Rhesus D blood group protein; Rhesus system D polypeptide; RhII; RhK562-II; RhPI; RHPII; RHXIII; truncated Rh blood group D antigen; truncated RhD antigen; truncated rhesus blood group D antigen; truncated rhesus D.

Gene ID

6007

Accession Number

NP_001121163.1

Reactivity

Homo sapiens|Human

Target

May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Detection Method

Colorimetric

Assay Principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Anti-Rh Blood Group, D Antigen Antibody (Anti-RHD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

3.12-200ng/mL

Sensitivity

< 1.27ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount RHD Microplate 96 Wells (12 x 8 Well strips) RHD Lyophilized Standard 2 100X RHD HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Component Amount Component Amount RHD Microplate 96 Wells (12 x 8 Well strips) RHD Microplate 96 Wells (12 x 8 Well strips) RHD Lyophilized Standard 2 RHD Lyophilized Standard 2 100X RHD HRP-Detector Anitbody 1 x 120 uL 100X RHD HRP-Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL TMB Substrate 1 x 9 mL TMB Substrate 1 x 9 mL Stop Solution 1 x 6 mL Stop Solution 1 x 6 mL