CXCL17 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level VEGF Co Regulated Chemokine 1 (VCC1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

6-Cys CXCL17; chemokine (C-X-C motif) ligand 17; C-X-C motif chemokine 17; Dcip1; dendritic cell and monocyte chemokine-like protein; DMC; UNQ473; VCC1; VCC-1; VEGF coregulated chemokine 1; VEGF co-regulated chemokine 1.

Chemokine that acts as chemoattractant for monocytes, macrophages and dendritic cells (PubMed:16455961, PubMed:23115081) . Plays a role in angiogenesis and possibly in the development of tumors (PubMed:16989774, PubMed:23115081) . Acts as an anti-inflammatory in the stomach (PubMed:23115081) . May play a role in the innate defense against infections (PubMed:17307946) . Activates the C-X-C chemokine receptor GPR35 to induce a rapid and transient rise in the level of intracellular calcium ions (PubMed:25411203) .

The microplate provided in this kit has been pre-coated with an antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VEGF Co Regulated Chemokine 1 (VCC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the VEGF Co Regulated Chemokine 1 (VCC1) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 95-103 99 EDTA Plasma (n=5) 78-103 97 Heparin Plasma (n=5) 78-94 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-103 99 Serum (n=5) 95-103 99 EDTA Plasma (n=5) 78-103 97 EDTA Plasma (n=5) 78-103 97 Heparin Plasma (n=5) 78-94 88 Heparin Plasma (n=5) 78-94 88

Component Amount CXCL17 Microplate 96 Wells (12 x 8 Well strips) CXCL17 Lyophilized Standard 2 100X CXCL17 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount CXCL17 Microplate 96 Wells (12 x 8 Well strips) CXCL17 Microplate 96 Wells (12 x 8 Well strips) CXCL17 Lyophilized Standard 2 CXCL17 Lyophilized Standard 2 100X CXCL17 Biotinylated Detector Anitbody 1 x 120 uL 100X CXCL17 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL