TPSAB1 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Tryptase (TPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Tryptase (TPS) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Epididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; TPS1; TPS2; TPSB1; TPSB2; tryptase alpha II; tryptase alpha/beta-1; tryptase alpha-1; tryptase beta I; tryptase beta-1; Tryptase beta-2; Tryptase II; tryptase-1; Tryptase-2; tryptase-I; tryptase-III.

The microplate provided in this kit has been pre-coated with an antibody specific to Tryptase (TPS) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tryptase (TPS) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tryptase (TPS) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 101 EDTA Plasma (n=5) 87-104 94 Heparin Plasma (n=5) 97-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 101 Serum (n=5) 96-103 101 EDTA Plasma (n=5) 87-104 94 EDTA Plasma (n=5) 87-104 94 Heparin Plasma (n=5) 97-105 101 Heparin Plasma (n=5) 97-105 101

Component Amount TPSAB1 Microplate 96 Wells (12 x 8 Well strips) TPSAB1 Lyophilized Standard 2 100X TPSAB1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount TPSAB1 Microplate 96 Wells (12 x 8 Well strips) TPSAB1 Microplate 96 Wells (12 x 8 Well strips) TPSAB1 Lyophilized Standard 2 TPSAB1 Lyophilized Standard 2 100X TPSAB1 Biotinylated Detector Anitbody 1 x 120 uL 100X TPSAB1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL