What is the catalog number for TGFB3 Chemi-Luminescent ELISA Kit (Rat)?

The catalog number for TGFB3 Chemi-Luminescent ELISA Kit (Rat) is OKCD03948-96W.

What sizes are available for TGFB3 Chemi-Luminescent ELISA Kit (Rat)?

TGFB3 Chemi-Luminescent ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is TGFB3 Chemi-Luminescent ELISA Kit (Rat) in stock?

TGFB3 Chemi-Luminescent ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

TGFB3 Chemi-Luminescent ELISA Kit (Rat)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Transforming Growth Factor Beta 3 (TGFb3) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Transforming growth factor, beta 3

Gene Aliases

TGF-B3; TGF-beta-3; transforming growth factor beta-3 proprotein.

Gene ID

25717

Accession Number

NP_037306

Reactivity

Rat|Rattus norvegicus

Target

Involved in embryogenesis and cell differentiation.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 3 (TGFb3) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 3 (TGFb3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transforming Growth Factor Beta 3 (TGFb3) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.37-1,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 88-98 94 EDTA Plasma (n=5) 89-97 93 Heparin Plasma (n=5) 93-102 97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 88-98 94 Serum (n=5) 88-98 94 EDTA Plasma (n=5) 89-97 93 EDTA Plasma (n=5) 89-97 93 Heparin Plasma (n=5) 93-102 97 Heparin Plasma (n=5) 93-102 97

Sensitivity

15.6 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Tgfb3 Microplate 96 Wells (12 x 8 Well strips) Tgfb3 Lyophilized Standard 2 100X Tgfb3 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Tgfb3 Microplate 96 Wells (12 x 8 Well strips) Tgfb3 Microplate 96 Wells (12 x 8 Well strips) Tgfb3 Lyophilized Standard 2 Tgfb3 Lyophilized Standard 2 100X Tgfb3 Biotinylated Detector Anitbody 1 x 120 uL 100X Tgfb3 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL