SERPINA7 Chemi-Luminescent ELISA Kit (Human)
Product Specifications
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Thyroxine Binding Globulin (TBG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Thyroxine Binding Globulin (TBG) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Serpin family A member 7
Gene Aliases
Mutant serpin family A member 7; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; T4-binding globulin; TBG; TBGQTL; thyroxin-binding globulin; thyroxine-binding globulin.
Gene ID
6906
Accession Number
NP_000345
Reactivity
Homo sapiens|Human
Target
Major thyroid hormone transport protein in serum.
Type
ELISA Kit
Applications
Chemiluminescent immunoassay
Detection Method
Chemiluminescent
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Thyroxine Binding Globulin (TBG) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thyroxine Binding Globulin (TBG) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thyroxine Binding Globulin (TBG) level in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.14-100ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 94-102 98 EDTA Plasma (n=5) 79-92 88 Heparin Plasma (n=5) 86-94 89 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94-102 98 Serum (n=5) 94-102 98 EDTA Plasma (n=5) 79-92 88 EDTA Plasma (n=5) 79-92 88 Heparin Plasma (n=5) 86-94 89 Heparin Plasma (n=5) 86-94 89
Sensitivity
0.14 ng/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount SERPINA7 Microplate 96 Wells (12 x 8 Well strips) SERPINA7 Lyophilized Standard 2 100X SERPINA7 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount SERPINA7 Microplate 96 Wells (12 x 8 Well strips) SERPINA7 Microplate 96 Wells (12 x 8 Well strips) SERPINA7 Lyophilized Standard 2 SERPINA7 Lyophilized Standard 2 100X SERPINA7 Biotinylated Detector Anitbody 1 x 120 uL 100X SERPINA7 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
SERPINA7
Protein Name
Thyroxine-binding globulin
Gene Name URL
SERPINA7
Nucleotide Accession Number
NM_000354
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