What is the catalog number for SELE Chemi-Luminescent ELISA Kit (Rat)?

The catalog number for SELE Chemi-Luminescent ELISA Kit (Rat) is OKCD03915-96W.

What sizes are available for SELE Chemi-Luminescent ELISA Kit (Rat)?

SELE Chemi-Luminescent ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is SELE Chemi-Luminescent ELISA Kit (Rat) in stock?

SELE Chemi-Luminescent ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

SELE Chemi-Luminescent ELISA Kit (Rat)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level E-selectin were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level E-selectin were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Selectin E

Gene Aliases

CD62 antigen-like family member E; ELAM-1; endothelial leukocyte adhesion molecule 1; E-selectin; LECAM2; leukocyte-endothelial cell adhesion molecule 2; selectin, endothelial cell.

Gene ID

25544

Accession Number

NP_620234

Reactivity

Rat|Rattus norvegicus

Target

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to E-selectin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to E-selectin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the E-selectin level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

2.74-2,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 88-96 93 EDTA Plasma (n=5) 89-104 93 Heparin Plasma (n=5) 94-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 88-96 93 Serum (n=5) 88-96 93 EDTA Plasma (n=5) 89-104 93 EDTA Plasma (n=5) 89-104 93 Heparin Plasma (n=5) 94-105 101 Heparin Plasma (n=5) 94-105 101

Sensitivity

2.74 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Sele Microplate 96 Wells (12 x 8 Well strips) Sele Lyophilized Standard 2 100X Sele Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Sele Microplate 96 Wells (12 x 8 Well strips) Sele Microplate 96 Wells (12 x 8 Well strips) Sele Lyophilized Standard 2 Sele Lyophilized Standard 2 100X Sele Biotinylated Detector Anitbody 1 x 120 uL 100X Sele Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL