What is the catalog number for GKN2 Chemi-Luminescent ELISA Kit (Rat)?

The catalog number for GKN2 Chemi-Luminescent ELISA Kit (Rat) is OKCD03836-96W.

What sizes are available for GKN2 Chemi-Luminescent ELISA Kit (Rat)?

GKN2 Chemi-Luminescent ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is GKN2 Chemi-Luminescent ELISA Kit (Rat) in stock?

GKN2 Chemi-Luminescent ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

GKN2 Chemi-Luminescent ELISA Kit (Rat)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Gastrokine 2 (GKN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Gastrokine 2 (GKN2) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Gastrokine 2

Gene Aliases

Blottin; gastrokine-2; RGD1311934.

Gene ID

297419

Accession Number

NP_001034775

Reactivity

Rat|Rattus norvegicus

Target

Gastrokine-2

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Gastrokine 2 (GKN2) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Gastrokine 2 (GKN2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Gastrokine 2 (GKN2) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Tissue homogenates and other biological fluids

Detection Range

1.37-1,000pg/mL

Sensitivity

15.6 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Gkn2 Microplate 96 Wells (12 x 8 Well strips) Gkn2 Lyophilized Standard 2 100X Gkn2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Gkn2 Microplate 96 Wells (12 x 8 Well strips) Gkn2 Microplate 96 Wells (12 x 8 Well strips) Gkn2 Lyophilized Standard 2 Gkn2 Lyophilized Standard 2 100X Gkn2 Biotinylated Detector Anitbody 1 x 120 uL 100X Gkn2 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL