NOS1 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Nitric Oxide Synthase 1, Neuronal (NOS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Nitric Oxide Synthase 1, Neuronal (NOS1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

BNOS; constitutive NOS; IHPS1; NC-NOS; neuronal NOS; nitric oxide synthase 1 (neuronal) ; nitric oxide synthase, brain; nNOS; N-NOS; NOS; NOS type I; peptidyl-cysteine S-nitrosylase NOS1.

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

The microplate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 1, Neuronal (NOS1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase 1, Neuronal (NOS1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nitric Oxide Synthase 1, Neuronal (NOS1) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 95-105 99 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 93-101 97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95-105 99 Serum (n=5) 95-105 99 EDTA Plasma (n=5) 95-103 99 EDTA Plasma (n=5) 95-103 99 Heparin Plasma (n=5) 93-101 97 Heparin Plasma (n=5) 93-101 97

Component Amount NOS1 Microplate 96 Wells (12 x 8 Well strips) NOS1 Lyophilized Standard 2 100X NOS1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount NOS1 Microplate 96 Wells (12 x 8 Well strips) NOS1 Microplate 96 Wells (12 x 8 Well strips) NOS1 Lyophilized Standard 2 NOS1 Lyophilized Standard 2 100X NOS1 Biotinylated Detector Anitbody 1 x 120 uL 100X NOS1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL