ENO1 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Enolase 1 (ENO1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Enolase 1 (ENO1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; alpha-enolase; C-myc promoter-binding protein; c-myc promoter-binding protein-1; ENO1L1; Enolase 1; enolase 1, (alpha) ; enolase-alpha; epididymis secretory protein Li 17; HEL-S-17; MBP-1; MPB1; MPB-1; MYC promoter-binding protein 1; NNE; non-neural enolase; phosphopyruvate hydratase; plasminogen-binding protein; PPH; tau-crystallin.

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.

The microplate provided in this kit has been pre-coated with an antibody specific to Enolase 1 (ENO1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Enolase 1 (ENO1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Enolase 1 (ENO1) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 94-101 97 EDTA Plasma (n=5) 78-102 90 Heparin Plasma (n=5) 86-101 94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94-101 97 Serum (n=5) 94-101 97 EDTA Plasma (n=5) 78-102 90 EDTA Plasma (n=5) 78-102 90 Heparin Plasma (n=5) 86-101 94 Heparin Plasma (n=5) 86-101 94

Component Amount ENO1 Microplate 96 Wells (12 x 8 Well strips) ENO1 Lyophilized Standard 2 100X ENO1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount ENO1 Microplate 96 Wells (12 x 8 Well strips) ENO1 Microplate 96 Wells (12 x 8 Well strips) ENO1 Lyophilized Standard 2 ENO1 Lyophilized Standard 2 100X ENO1 Biotinylated Detector Anitbody 1 x 120 uL 100X ENO1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL