What is the catalog number for NBL1 Chemi-Luminescent ELISA Kit (Human)?

The catalog number for NBL1 Chemi-Luminescent ELISA Kit (Human) is OKCD03824-96W.

What sizes are available for NBL1 Chemi-Luminescent ELISA Kit (Human)?

NBL1 Chemi-Luminescent ELISA Kit (Human) is available in the following size(s): 96 Well.

Is NBL1 Chemi-Luminescent ELISA Kit (Human) in stock?

NBL1 Chemi-Luminescent ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

NBL1 Chemi-Luminescent ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

MICOS10-NBL1 readthrough|NBL1, DAN family BMP antagonist

Gene Aliases

C1orf151-NBL1; C1orf151-NBL1 read-through protein; D1S1733E; DAN; DAN domain family member 1; DAND1; differential screening-selected gene aberrant in neuroblastoma; MICOS10-NBL1 read-through protein; MINOS1-NBL1; MINOS1-NBL1 readthrough; NB; NBL1; neuroblastoma 1, DAN family BMP antagonist; neuroblastoma candidate region, suppression of tumorigenicity 1; neuroblastoma suppressor of tumorigenicity 1; NO3; Protein N03; Zinc finger protein DAN.

Gene ID

100532736|4681

Accession Number

NP_001191013

Reactivity

Homo sapiens|Human

Target

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

27.4-20,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 84-104 94 EDTA Plasma (n=5) 86-94 91 Heparin Plasma (n=5) 78-99 91 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 84-104 94 Serum (n=5) 84-104 94 EDTA Plasma (n=5) 86-94 91 EDTA Plasma (n=5) 86-94 91 Heparin Plasma (n=5) 78-99 91 Heparin Plasma (n=5) 78-99 91

Sensitivity

0.0274 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount NBL1 Microplate 96 Wells (12 x 8 Well strips) NBL1 Lyophilized Standard 2 100X NBL1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount NBL1 Microplate 96 Wells (12 x 8 Well strips) NBL1 Microplate 96 Wells (12 x 8 Well strips) NBL1 Lyophilized Standard 2 NBL1 Lyophilized Standard 2 100X NBL1 Biotinylated Detector Anitbody 1 x 120 uL 100X NBL1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL