CXCL1 Chemi-Luminescent ELISA Kit (Mouse)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Alpha-chemokine; C-X-C motif chemokine 1; F; Fsp; Gr; gro; Gro1; GRO1 oncogene; growth-regulated alpha protein; KC; KC/GR) -alpha; KC/GRO-alpha; Mg; Mgsa; N5; N51; platelet-derived growth factor-inducible protein KC; Scyb; Scyb1; secretory protein N51.

Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation (By similarity) . Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. KC (5-72) shows a highly enhanced hematopoietic activity.

The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine (C-X-C Motif) Ligand 1 (CXCL1) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 88-103 99 EDTA Plasma (n=5) 94-104 101 Heparin Plasma (n=5) 90-104 96 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 88-103 99 Serum (n=5) 88-103 99 EDTA Plasma (n=5) 94-104 101 EDTA Plasma (n=5) 94-104 101 Heparin Plasma (n=5) 90-104 96 Heparin Plasma (n=5) 90-104 96

Component Amount Cxcl1 Microplate 96 Wells (12 x 8 Well strips) Cxcl1 Lyophilized Standard 2 100X Cxcl1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Cxcl1 Microplate 96 Wells (12 x 8 Well strips) Cxcl1 Microplate 96 Wells (12 x 8 Well strips) Cxcl1 Lyophilized Standard 2 Cxcl1 Lyophilized Standard 2 100X Cxcl1 Biotinylated Detector Anitbody 1 x 120 uL 100X Cxcl1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL