MMP1 Chemi-Luminescent ELISA Kit (Horse)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Matrix Metalloproteinase 1 (MMP1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Matrix metallopeptidase 1

Gene Aliases

Interstitial collagenase; Interstitial collagenase-like protein; matrix metalloproteinase-1; MMP-1.

Gene ID

100033896

Accession Number

NP_001075316

Reactivity

Equus caballus|Horse

Target

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 1 (MMP1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 1 (MMP1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 1 (MMP1) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

13.7-10,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 101 EDTA Plasma (n=5) 93-101 96 Heparin Plasma (n=5) 80-92 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98-105 101 Serum (n=5) 98-105 101 EDTA Plasma (n=5) 93-101 96 EDTA Plasma (n=5) 93-101 96 Heparin Plasma (n=5) 80-92 88 Heparin Plasma (n=5) 80-92 88

Sensitivity

0.156 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Mmp1 Microplate 96 Wells (12 x 8 Well strips) Mmp1 Lyophilized Standard 2 100X Mmp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Mmp1 Microplate 96 Wells (12 x 8 Well strips) Mmp1 Microplate 96 Wells (12 x 8 Well strips) Mmp1 Lyophilized Standard 2 Mmp1 Lyophilized Standard 2 100X Mmp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Mmp1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL