What is the catalog number for FASN Chemi-Luminescent ELISA Kit (Chicken)?

The catalog number for FASN Chemi-Luminescent ELISA Kit (Chicken) is OKCD03754-96W.

What sizes are available for FASN Chemi-Luminescent ELISA Kit (Chicken)?

FASN Chemi-Luminescent ELISA Kit (Chicken) is available in the following size(s): 96 Well.

Is FASN Chemi-Luminescent ELISA Kit (Chicken) in stock?

FASN Chemi-Luminescent ELISA Kit (Chicken) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.

FASN Chemi-Luminescent ELISA Kit (Chicken)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Aliases

FAS, Fatty acid synthase

Gene ID

396061

Accession Number

NP_990486

Reactivity

Chicken|Gallus gallus

Target

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Fatty Acid Synthase (FASN) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fatty Acid Synthase (FASN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fatty Acid Synthase (FASN) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

5.49-4,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 93-101 96 EDTA Plasma (n=5) 86-103 89 Heparin Plasma (n=5) 83-101 92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93-101 96 Serum (n=5) 93-101 96 EDTA Plasma (n=5) 86-103 89 EDTA Plasma (n=5) 86-103 89 Heparin Plasma (n=5) 83-101 92 Heparin Plasma (n=5) 83-101 92

Sensitivity

2.5 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Fasn Microplate 96 Wells (12 x 8 Well strips) Fasn Lyophilized Standard 2 100X Fasn Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Fasn Microplate 96 Wells (12 x 8 Well strips) Fasn Microplate 96 Wells (12 x 8 Well strips) Fasn Lyophilized Standard 2 Fasn Lyophilized Standard 2 100X Fasn Biotinylated Detector Anitbody 1 x 120 uL 100X Fasn Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL