What is the catalog number for LTF Chemi-Luminescent ELISA Kit (Bovine)?

The catalog number for LTF Chemi-Luminescent ELISA Kit (Bovine) is OKCD03730-96W.

What sizes are available for LTF Chemi-Luminescent ELISA Kit (Bovine)?

LTF Chemi-Luminescent ELISA Kit (Bovine) is available in the following size(s): 96 Well.

Is LTF Chemi-Luminescent ELISA Kit (Bovine) in stock?

LTF Chemi-Luminescent ELISA Kit (Bovine) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

LTF Chemi-Luminescent ELISA Kit (Bovine)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Lactotransferrin

Gene Aliases

Lactoferrin; lactotransferrin; Lf.

Gene ID

280846

Accession Number

NP_851341

Reactivity

Bos taurus|Bovine

Target

Transferrins are iron binding transport proteins which can bind two Fe (3+) ions in association with the binding of an anion, usually bicarbonate.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lactoferrin (LTF) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates, saliva, milk, cell culture supernates and other biological fluids

Detection Range

0.41-300ng/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 91-99 96 EDTA Plasma (n=5) 80-93 85 Heparin Plasma (n=5) 84-92 88 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 91-99 96 Serum (n=5) 91-99 96 EDTA Plasma (n=5) 80-93 85 EDTA Plasma (n=5) 80-93 85 Heparin Plasma (n=5) 84-92 88 Heparin Plasma (n=5) 84-92 88

Sensitivity

0.41 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount Ltf Microplate 96 Wells (12 x 8 Well strips) Ltf Lyophilized Standard 2 100X Ltf Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Ltf Microplate 96 Wells (12 x 8 Well strips) Ltf Microplate 96 Wells (12 x 8 Well strips) Ltf Lyophilized Standard 2 Ltf Lyophilized Standard 2 100X Ltf Biotinylated Detector Anitbody 1 x 120 uL 100X Ltf Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL