CXCL11 Chemi-Luminescent ELISA Kit (Human)

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; C-X-C motif chemokine 11; H174; interferon gamma-inducible protein 9; interferon-inducible T-cell alpha chemoattractant; IP9; IP-9; I-TAC; SCYB11; SCYB9B; small inducible cytokine B11; small inducible cytokine subfamily B (Cys-X-Cys), member 11; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11.

Chemotactic for interleukin-activated T-cells but not unstimulated T-cells, neutrophils or monocytes. Induces calcium release in activated T-cells. Binds to CXCR3. May play an important role in CNS diseases which involve T-cell recruitment. May play a role in skin immune responses.

The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) level in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Matrix Recovery range (%) Average (%) Serum (n=5) 85-101 96 EDTA Plasma (n=5) 92-99 96 Heparin Plasma (n=5) 98-105 101 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 85-101 96 Serum (n=5) 85-101 96 EDTA Plasma (n=5) 92-99 96 EDTA Plasma (n=5) 92-99 96 Heparin Plasma (n=5) 98-105 101 Heparin Plasma (n=5) 98-105 101

Component Amount CXCL11 Microplate 96 Wells (12 x 8 Well strips) CXCL11 Lyophilized Standard 2 100X CXCL11 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount CXCL11 Microplate 96 Wells (12 x 8 Well strips) CXCL11 Microplate 96 Wells (12 x 8 Well strips) CXCL11 Lyophilized Standard 2 CXCL11 Lyophilized Standard 2 100X CXCL11 Biotinylated Detector Anitbody 1 x 120 uL 100X CXCL11 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL