IL6 Chemi-Luminescent ELISA Kit (Pig)
Product Specifications
Reproducibility
Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
CAS Number
7732-18-5
Gene Name
Interleukin 6|interleukin-6
Gene Aliases
IL-6; interleukin 6 (interferon, beta 2) ; interleukin-6; interleukin-6 protein.
Gene ID
100628202|399500
Accession Number
NP_001239358
Reactivity
Porcine|Sus scrofa
Target
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T (H) 17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity) .
Type
ELISA Kit
Applications
Chemiluminescent immunoassay
Detection Method
Chemiluminescent
Assay Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 (IL6) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 6 (IL6) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 6 (IL6) level in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.37-1,000pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 87-102 91 EDTA Plasma (n=5) 90-99 94 Heparin Plasma (n=5) 81-94 89 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 87-102 91 Serum (n=5) 87-102 91 EDTA Plasma (n=5) 90-99 94 EDTA Plasma (n=5) 90-99 94 Heparin Plasma (n=5) 81-94 89 Heparin Plasma (n=5) 81-94 89
Sensitivity
1.37 pg/mL
Reconstitution
2°C to 8°C|-20°C
Components
Component Amount Il6 Microplate 96 Wells (12 x 8 Well strips) Il6 Lyophilized Standard 2 100X Il6 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount Il6 Microplate 96 Wells (12 x 8 Well strips) Il6 Microplate 96 Wells (12 x 8 Well strips) Il6 Lyophilized Standard 2 Il6 Lyophilized Standard 2 100X Il6 Biotinylated Detector Anitbody 1 x 120 uL 100X Il6 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL
NCBI Gene Symbol
IL6|IL-6
Protein Name
Interleukin-6
Gene Name URL
IL6|IL-6
Nucleotide Accession Number
NM_001252429
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