What is the catalog number for IFNA21 Chemi-Luminescent ELISA Kit (Human)?

The catalog number for IFNA21 Chemi-Luminescent ELISA Kit (Human) is OKCD03587-96W.

What sizes are available for IFNA21 Chemi-Luminescent ELISA Kit (Human)?

IFNA21 Chemi-Luminescent ELISA Kit (Human) is available in the following size(s): 96 Well.

Is IFNA21 Chemi-Luminescent ELISA Kit (Human) in stock?

IFNA21 Chemi-Luminescent ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

IFNA21 Chemi-Luminescent ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Interferon Alpha 21 (IFNa21) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Interferon Alpha 21 (IFNa21) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Interferon alpha 21

Gene Aliases

IFN-alpha-21; IFN-alphaI; interferon alpha-21; interferon alpha-F; LeIF F; leIF-F; leukocyte interferon protein.

Gene ID

3452

Accession Number

NP_002166

Reactivity

Homo sapiens|Human

Target

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 21 (IFNa21) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha 21 (IFNa21) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 21 (IFNa21) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.37-1,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 80-91 84 EDTA Plasma (n=5) 79-96 83 Heparin Plasma (n=5) 78-101 83 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 80-91 84 Serum (n=5) 80-91 84 EDTA Plasma (n=5) 79-96 83 EDTA Plasma (n=5) 79-96 83 Heparin Plasma (n=5) 78-101 83 Heparin Plasma (n=5) 78-101 83

Sensitivity

15.6 pg/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount IFNA21 Microplate 96 Wells (12 x 8 Well strips) IFNA21 Lyophilized Standard 2 100X IFNA21 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount IFNA21 Microplate 96 Wells (12 x 8 Well strips) IFNA21 Microplate 96 Wells (12 x 8 Well strips) IFNA21 Lyophilized Standard 2 IFNA21 Lyophilized Standard 2 100X IFNA21 Biotinylated Detector Anitbody 1 x 120 uL 100X IFNA21 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL