What is the catalog number for ICAM1 Chemi-Luminescent ELISA Kit (Human)?

The catalog number for ICAM1 Chemi-Luminescent ELISA Kit (Human) is OKCD03569-96W.

What sizes are available for ICAM1 Chemi-Luminescent ELISA Kit (Human)?

ICAM1 Chemi-Luminescent ELISA Kit (Human) is available in the following size(s): 96 Well.

Is ICAM1 Chemi-Luminescent ELISA Kit (Human) in stock?

ICAM1 Chemi-Luminescent ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

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What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

ICAM1 Chemi-Luminescent ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

CAS Number

7732-18-5

Gene Name

Intercellular adhesion molecule 1

Gene Aliases

BB2; CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; ICAM-1; intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor; P3.58.

Gene ID

3383

Accession Number

NP_000192

Reactivity

Homo sapiens|Human

Target

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) . During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

Type

ELISA Kit

Applications

Chemiluminescent immunoassay

Detection Method

Chemiluminescent

Assay Principle

The microplate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1) . Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Intercellular Adhesion Molecule 1 (ICAM1) level in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

6.86-5,000pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 99 EDTA Plasma (n=5) 93-105 102 Heparin Plasma (n=5) 88-96 91 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96-103 99 Serum (n=5) 96-103 99 EDTA Plasma (n=5) 93-105 102 EDTA Plasma (n=5) 93-105 102 Heparin Plasma (n=5) 88-96 91 Heparin Plasma (n=5) 88-96 91

Sensitivity

0.0137 ng/mL

Reconstitution

2°C to 8°C|-20°C

Components

Component Amount ICAM1 Microplate 96 Wells (12 x 8 Well strips) ICAM1 Lyophilized Standard 2 100X ICAM1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Component Amount Component Amount ICAM1 Microplate 96 Wells (12 x 8 Well strips) ICAM1 Microplate 96 Wells (12 x 8 Well strips) ICAM1 Lyophilized Standard 2 ICAM1 Lyophilized Standard 2 100X ICAM1 Biotinylated Detector Anitbody 1 x 120 uL 100X ICAM1 Biotinylated Detector Anitbody 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL 100X Avidin-HRP Conjugate 1 x 120 uL Standard Diluent 1 x 20 mL Standard Diluent 1 x 20 mL Detector Antibody Diluent 1 x 12 mL Detector Antibody Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL Conjugate Diluent 1 x 12 mL 30X Wash Buffer 1 x 20 mL 30X Wash Buffer 1 x 20 mL 100X Luminol Substrate 1 x 2 mL 100X Luminol Substrate 1 x 2 mL Substrate Diluent 1 x 20 mL Substrate Diluent 1 x 20 mL